A method for the analysis of common mycotoxins in peanuts and oils was developed using ultra performance liquid chromatography with tandem mass spectrometry(UPLC - MS/MS), based on isotope dilution and gel permeation chromatography (GPC) purification. The homogenized sample was spiked with U - [13C17]-aflatoxin B1 and U - [13C15]-deoxynivalenol), and extracted with acetonitrile-water(84: 16, by volume), then purified by GPC. The targeted compounds were detected by MS/MS system with eletrospray ionization(ESI) under muti-reaction monitoring(MRM) mode and quantified by the internal standard calibration curve of isotope dilution technique. The result indicated that the LOQ of 12 positive ions ranged from 0. 02 μg/kg to 0. 4 μg/kg, the LOQ of 6 negative ions ranged from 0.06 μg/kg to 1.0 μg/kg. Meanwhile, the high correlation coefficents(r≥O. 996)of 18 mycotoxins were obtained within their respective linear ranges. The average recoveries at low,intermediate and high spiked levels ranged from 80％ to 96％ with relative standard deviations(RSD)of 10. 5％ - 19.6％. This method was suitable for the identification and quantification of multi-compontent mycotoxin contaminants in complex matrixes with advantages of simple pretreatment, good purification and high sensitivity.%采用同位素稀释法并结合凝胶色谱净化技术,建立了花生、粮油中18种常见真菌毒素污染的超高效液相色谱串联质谱分析方法.样品中添加同位素内标U-[13C17]-黄曲霉毒素 B1和U-[13C15]-脱氧雪腐镰刀菌烯醇,经乙腈-水溶液(84:16,体积比)均质提取,凝胶渗透色谱净化,Waters ACQUITY UPLCTM BEH C18色谱柱分离,串联四极杆质谱多反应离子监测方式检测,同位素稀释内标法定量.结果表明,18种真菌毒素在各自的线性范围内线性关系良好,相关系数均不低于0.996;其中12种化合物的定量下限为0.02～0.4 μg/kg,6种负离子的定量下限为0.06～1.0 μg/kg;低、中、高3个加标水平的回收率为80%～96%,相对标准偏差为10.5%～19.6%.该方法具有前处理简单、净化效果好、灵敏度高的优点,适用于复杂基质样品中多组分真菌毒素残留的确认和定量检测.
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