衍生化气相色谱-质谱法测定植物油中植物甾醇

摘要

植物油样品经氢氧化钾甲醇溶液皂化后,硅烷化衍生,采用气相色谱-质谱( GC - MS)法建立了植物油中菜籽甾醇、菜油甾醇、豆甾醇、β-谷甾醇4种植物甾醇含量的分析方法,研究了各植物甾醇衍生物的质谱特征和断裂机理.该方法方便快捷、准确可靠,检出限为0.01 g/kg,用于植物油样品中植物甾醇的组成和含量测定,结果满意.%A gas chromatography - mass spectrometric ( GC - MS) method was established for the determination of four main kinds of phytosterols ( e. g. brassicasterol, campesterol, stigmasterol and β-sitosterol) in vegetable oils. The phytosterols were transformed to the corresponding TMS derivatives and subsequently analyzed by GC - MS. The mass spectrometric fragmentation rule and mass characteristic ions of the derivatives were also studied. The method is accurate, reliable and convenient, and the detection limit was 0. 01 g/kg. It was applied in the qualitative and quantitative determination of phytosterols in vegetable oils with satisfactory results.