液相色谱-串联质谱法测定尿液中的泛酸

摘要

该文建立了检测尿液中泛酸含量的液相色谱-串联质谱(HPLC-MS/MS)方法,尿液经过离心、稀释后,采用ACPUITY UPLC SS T3(2.1 mm ×l00 mm,1.8lμm)色谱柱进行分离,电喷雾正离子模式电离,多反应监测模式进行检测,方法的线性关系良好(r =0.999 3),方法检出限为0.46 ng/mL,回收率为87.9％～95.3％,相对标准偏差(RSD)为2.5％～13.0％.该方法具有灵敏度高、分析时间短等特点,可用于尿液中泛酸含量的分析.%A liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method for the determination of pantothenic acid in urine was developed and validated.After centrifugation and dilution,the sample was separated on an ACPUITY UPLC HSS T3(2.1 mm× 100 mm,1.8 μm) column produced by the Waters corporation,and ionized in electro spray positive ion mode,then detected using multi-reaction monitoring mode.The calibration curve has a good linear range with a correlation coefficient(r) of 0.999 3.The limit of detection is 0.46 ng/mL,the recoveries are in the range of 87.9％-95.3％,and the RSDs are in the range of 2.5％-13.0％.The method has the advantages of high sensitivity and short analysis time,and could be used to analyze pantothenic acid in human urine.