Determination of Isoprostanes in Urine from Smokers and Nonsmokers by Liquid Chromatography-Tandem Mass Spectrometry

摘要

Isoprostanes are prostaglandin-like compounds produced in vivo by non-enzymatic free-radical-induced peroxidation of unsaturated fatty acids. Quantification of these compounds has been proposed as a useful index of lipid peroxidation and oxidative stress in vivo. Elevated levels of an extensively studied isomer, 8-iso-prostaglandin F_(2(alpha)) (iPF_(2(alpha))-III) and its metabolite, 2,3-dinor-8-iso-prostaglandin F_(2(alpha)) (2,3-dinor-iPF_(2(alpha))-III), have been reported in smokers as compared to nonsmokers [1].