高效液相色谱/二极管阵列法快速测定白酒、配制酒与葡萄酒中5种人工合成甜味剂

摘要

A high performance liquid chromatographic (HPLC) method was developed for the rapid determination of five synthetic sweeteners,including acesulfame-K (AK),saccharin sodium (SA),aspartame(ASP),alitame(ALT) and neotame(NTM) in white spirit,mixed liquor and wine.The HPLC separation was carried out on a C1s column.Several parameters,including the composition and gradient elution program of the mobile phase,column temperature and optimum detection wavelength of diode-array detector(DAD) were optimized for improving the chromatographic performance and the sensitivity of determination.The results demonstrated that the separation could be completed within 15 min by gradient elution with acetonitrile and 0.02 mol/L ammonium sulfate solution (pH 4.4) as mobile phase.The column temperature was set at 40 ℃.The calibration curves of five synthetic sweeteners were linear in the range of 4-200 mg/L with correlation coefficients more than 0.999.The recoveries were in the range of 95.2％-103.2％,with relative standard deviations (n =5) not more than 3.4％.The experimental results indicated that the established method was simple,efficient and sensitive,and was suitable for the determination of five synthetic sweeteners in white spirit,mixed liquor and wine.%建立了一种快速检测白酒、配制酒和葡萄酒中安赛蜜、糖精钠、阿斯巴甜、阿力甜及纽甜5种人工合成甜味剂的高效液相色谱分析方法.采用C18柱为分离柱,对流动相的组成和洗脱方式、检测波长等参数进行优化.结果表明,以乙腈和0.02 mol/L硫酸铵(pH 4.4)溶液为流动相,梯度洗脱,柱温40 ℃,可使5种人工合成甜味剂在15 min内实现基线分离.最佳检测波长下,5种甜味剂在4～ 200 mg/L浓度范围内呈良好的线性关系,相关系数均大于0.999.样品加标回收率为95.2％ ～ 103.2％,相对标准偏差(n=5)均不大于3.4％.该方法简便、快捷、准确、灵敏度较高,适用于白酒、配制酒和葡萄酒等各类酒中5种人工合成甜味剂的快速检测.