Rapid separation and simultaneous quantitative determination of 13 constituents in Psoraleae Fructus by a single marker using high‐performance liquid chromatography with diode array detection

Zhang Yimin; Chen Zhiyong; Xu Xiaokun; Zhou Qiang; Liu Xiaolong; Liao Liping; Zhang Zijia; Wang Zhengtao

摘要

Abstract >Psoraleae Fructus is one of the most popular traditional Chinese medicines. Coumarins, flavonoids, and meroterpenes are the main contributors to the biological activity of Psoraleae Fructus. In this study, a new method for the quality control of Psoraleae Fructus was developed, through the quantitative analysis of multicomponents by single marker with diode array detector. Thirteen components, including psoralenoside, isopsoralenoside, psoralen, isopsoralen, psoralidin, neobavaisoflavone, bavachin, corylin, isobavachalcone, corylifol A, bavachinin, bavachalcone, and bakuchiol were rapidly separated and identified within 12?min by the newly developed method. The feasibility and reliability of this method were corroborated. The method was also compared to the external standard method and detection by corona charged aerosol detector. The results of percent difference (%) and cos (θ) have shown that there were no significant differences observed between the quantitative analysis of multicomponents by single marker and external standard method analyses; psoralen and isopsoralen were undetectable with the corona charged aerosol detector due to their but the sensitivity for all the compounds except bakuchiol detected by corona charged aerosol detector are higher than those obtained by diode array detector. In addition, the newly method developed was applied to the quality evaluation of Chinese patent medicines containing Psoraleae Fructus. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > psoraleae fructus是最受欢迎的中药之一。香豆素，黄酮类化合物和梅特彼得斯是牛肝菌果实生物活性的主要贡献者。在本研究中，通过用二极管阵列检测器的单个标记进行多组分的定量分析，开发了一种新的用于Psoraleae Fructus的质量控制的新方法。包括牛油皂苷，患有牛油苷，牛油苷，牛油醛，患有患有牛油苷，巴拉昔洛，巴卡辛，甲酰胺，甲磺酰甲酰胺，罗西林A，Bavachinin，Bavachalcone和Bakuchiol，并通过新开发的方法在12〜min内鉴定。这种方法的可行性和可靠性被证实。该方法也与外部标准方法进行比较，并通过电晕带电的气溶胶探测器检测。百分比（％）和COS（θ）的结果表明，通过单个标记和外标方法分析，多组分的定量分析之间观察到的显着差异;由于透视电晕的气溶胶探测器检测到除了由二极管阵列检测器获得的所有化合物之外，对电晕带电的气溶胶检测器无法检测到Psoralen和Isoxsoralen。此外，将新方法开发应用于含有Psoraleae Fructus的中国专利药物的质量评估。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2017年第21期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Yimin&option=202" target="_blank" rel="nofollow">Zhang Yimin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Zhiyong&option=202" target="_blank" rel="nofollow">Chen Zhiyong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xu Xiaokun&option=202" target="_blank" rel="nofollow">Xu Xiaokun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou Qiang&option=202" target="_blank" rel="nofollow">Zhou Qiang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Xiaolong&option=202" target="_blank" rel="nofollow">Liu Xiaolong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liao Liping&option=202" target="_blank" rel="nofollow">Liao Liping;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Zijia&option=202" target="_blank" rel="nofollow">Zhang Zijia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Zhengtao&option=202" target="_blank" rel="nofollow">Wang Zhengtao;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> <p>The MOE Key Laboratory for Standardization of Chinese Medicines and The Shanghai Key Laboratory for Compound Chinese MedicinesShanghai University of Traditional Chinese MedicineShanghai People's Republic of China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=charged aerosol detector&option=203" rel="nofollow">charged aerosol detector;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=diode array detector&option=203" rel="nofollow">diode array detector;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Psoraleae Fructus&option=203" rel="nofollow">Psoraleae Fructus;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=relative conversion factors&option=203" rel="nofollow">relative conversion factors;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=traditional Chinese medicine&option=203" rel="nofollow">traditional Chinese medicine;</a> </p> <div class="translation"> 机译：带电气溶胶探测器;二极管阵列探测器;psoraleae fructus;相对转换因子;中医; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025846254.html">Rapid separation and simultaneous quantitative determination of 13 constituents in Psoraleae Fructus by a single marker using high‐performance liquid chromatography with diode array detection</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Yimin&option=202" target="_blank" rel="nofollow" class="tuijian_auth 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href="/academic-conference-cn_meeting-16506_thesis/020221654744.html">反相高效液相色谱法-二极管阵列检测器检测复方关节炎胶囊中维生素B1、吡罗昔康和醋酸泼尼松成分</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈坚&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈坚</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈乃江&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈乃江</a> <span> <a href="/conference-cn-16506/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2009年中国药学大会暨第九届中国药师周 </a> <span> <span> . 2009</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314060124.html">高效液相色谱-二极管阵列/荧光检测器串联法同时测定化妆品中8种性激素的研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=程俊&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 程俊</a> <span> . 2016</span> </span> </div> </li> 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<span>机译：多维高效液相色谱（star7）分离技术用于定量测定550s F（288s c）上的重烃流中的7个馏分 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130473338823.html">HIGH PERFORMANCE LIQUID CHROMATOGRAPHY QUANTITATIVE ANALYTICAL METHOD AND APPARATUS USING MULTI-WAVELENGTH SIMULTANEOUS DETECTION</a> <b>[P]</b> . <span> 外国专利：  JPS6024447A </span> <span> . 1985-02-07</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高效液相色谱定量分析的多波长同时检测方法及装置 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130473255708.html">MULTIPLEX QUALITATIVE AND QUANTITATIVE DETERMINATION OF VITAMINES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND FLUORESCENT DETECTION METHOD</a> <b>[P]</b> . <span> 外国专利：  JPS6089750A </span> <span> . 1985-05-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: 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