不同助溶剂作用下桉叶油对痤疮丙酸杆菌的作用

摘要

Objective To observe eucalyptus oil with different cosolvent on Propionibacterium acnes(P. acnes) and provide certain references for the treatment of acne, at the same time provide the support for the development and utilization of eucalyptus oil resources. Methods The eucalyptus oil with different cosolvent on bacillus bacteriostatic ring diameter and the minimum bacteriostasis concentration of acne propionic acid were observed by using tablet Oxford cup method and test tube method. Results 80% pure eucalyptus oil, 40% eucalyptus oil ethanol solution and 40% eucalyptus oil acetone solution show completely bacteriostatic action on P. acnes, there is no bacterial growth on tablet. The average bacteriostatic circle positive control medicine (16 ug/mL erythromycin) on P. acnes was 29.44 mm; Cosolvent 95% ethanol and acetone didn't show bacteriostatic action on P. acnes; Blank control (distilled water) had no bacteriostatic action on P. acnes. When acetone as cosolvent, the minimal inhibitory concentrations (MIC) of eucalyptus oil was 0.625%; When 95% ethanol as cosolvent, the MIC of eucalyptus oil was 1.25%. Conclusion Eucalyptus oil show good bacteriostasis on P. acnes. The cosolvent 95%ethanol and acetone didn't exhibit bacteriostatic action. Cosolvent has a certain influence on the MIC of eucalyptus oil, when acetone as cosolvent the minimum bacteriostasis concentration was lower than 95% ethanol as cosolvent.%目的 观察不同助溶剂作用下桉叶油对痤疮丙酸杆菌的作用,为痤疮的治疗提供一定实验参考,同时为开发利用桉叶油资源提供支撑. 方法 采用平板牛津杯法及试管法,观察桉叶油在不同助溶剂作用条件下对痤疮丙酸杆菌的抑菌环直径及最低抑菌浓度. 结果 纯桉叶油(80%浓度)、桉叶油乙醇溶液(40%浓度)及桉叶油丙酮溶液(40%浓度)对痤疮丙酸杆菌能表现出完全抑菌作用, 平板上无细菌生长, 阳性对照药 (16 μg/mL 红霉素) 对痤疮丙酸杆菌平均抑菌圈直径为29.44 mm;助溶剂95%乙醇及丙酮对痤疮杆菌无抑菌作用;空白对照(蒸馏水)对痤疮丙酸杆菌无抑菌作用;当以丙酮为助溶剂时,桉叶油的最低抑菌浓度为浓度0.625%;当以95%乙醇为助溶剂时,桉叶油的最低抑菌浓度为1.25%.结论 桉叶油对痤疮丙酸杆菌有较好的抑菌作用;助溶剂95%乙醇、丙酮对痤疮丙酸杆菌无抑菌作用;助溶剂对桉叶油的最低抑菌浓度有一定的影响,丙酮为助溶剂时较95%乙醇为助溶剂时最低抑菌浓度低.