采用平衡透析法研究雷公藤甲素与人血清白蛋白的结合率，结果表明当雷公藤甲素的浓度分别为3．60，7．20和36．0μg／mL时，其与人血清白蛋白的结合率分别为（24．2±2．0）％，（18．4±0．4）％和（6．30±0．3）％．应用荧光淬灭法研究雷公藤甲素与人血清白蛋白间的相互作用机制，结果表明TP与HSA之间的结合为静态淬灭过程，在300，305和310 K温度下结合常数分别为1．3×104，7．8×103和3．8×103 L／mol，主要作用力为范德华力．%In the present work,the binding rate of triptolide ( TP) with human serum albumin ( HSA) was studied by equilibrium dialysis method. The results indicated that the drug⁃protein binding rates were (24. 2 ± 2. 0)%,(18. 4 ± 0. 4)% and (6. 30 ± 0. 3)% at the TP concentrations of 3. 60,7. 20 and 36. 0 μg/mL, respectively. Additionally,the binding mechanism of TP and HSA was investigated by fluorescence quenching method. The results showed that the binding constants at the temperatures of 300,305 and 310 K were 1. 3 × 104 , 7. 8 × 103 and 3. 8 × 103 L/mol,respectively. The binding between TP and HSA is a static quenching process and the main binding force between them is Van der Waals’ force.
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