摘要：
目的 测定胃炎灵颗粒中栀子苷、芍药苷和柚皮苷在牛血清白蛋白、大鼠血浆、人血浆的蛋白结合率.方法 采用平衡透析法测定胃炎灵颗粒中3种成分在牛血清白蛋白、大鼠血浆、人血浆的蛋白结合率;采用HPLC法测定透析内、外液中3种成分的质量浓度,计算血浆蛋白结合率.结果 栀子苷、芍药苷和柚皮苷的线性关系、精密度、提取回收率和稳定性均符合方法学要求;胃炎灵颗粒在低、中、高浓度(5.084、10.04、20.08 g/L)时,栀子苷与牛血清白蛋白的蛋白结合率分别为(23.77±13.39)％、(10.75±1.34)％、(5.82±6.53)％;芍药苷的分别为(51.81±6.53)％、(30.12±6.61)％、(21.39±9.45)％;柚皮苷的分别为(70.21±3.31)％、(67.61±1.31)％、(56.00±3.46)％;栀子苷与大鼠血浆的蛋白结合率分别为(71.56±3.00)％、(43.56±12.22)％、(40.63±4.73)％;芍药苷的分别为(55.72±1.60)％、(47.59±6.33)％、(40.07±0.72)％;柚皮苷的分别为(85.85±3.53)％、(85.38±0.99)％、(79.99±2.57)％;栀子苷与人血浆中的蛋白结合率分别为(13.56±2.40)％、(3.02±2.41)％、(5.82±2.08)％;芍药苷的分别为(9.49±5.23)％、(5.01±1.1)％、(3.98±1.54)％;柚皮苷的分别为(25.04±2.61)％、(26.09±13.82)％、(20.20±2.24)％.结论 胃炎灵颗粒在5.084、10.04、20.08 g/L时,栀子苷、芍药苷和柚皮苷的蛋白结合率表现为大鼠血浆＞牛血清白蛋白＞人血浆,种属差异明显.%Objective To study the protein binding rates ofgardenioside,paeoniflorin and naringin in Weiyanling granules in bovine serum albumin,rat plasma and human plasma.Methods The equilibrium dialysis method was carried to determine the binding-rates of three components in Weiyanling granules in bovine serum albumin,rat plasma and human plasma.The HPLC method was used to determine the mass concentration of three components in weiyanling granules in inner and outer dialysis and to calculate the plasma protein-binding rates.Results The linear relationship,precision,extraction recovery and stability of gardenoside,paeoniflorin and naringin all conformed to the methodological requirements.Within the low,middle and high concentration (5.084,10.04 and 20.08 g/L) of Weiyanling granules,the protein-binding rates of gardenioside and bovine serum albumin were 23.77％ ± 13.39％,10.75％ ± 1.34％,5.82％ ± 6.53％,respectively;the protein-binding rates of paeoniflorin were 51.81％ ± 6.53％,30.12％ ± 6.61％,21.39％ ± 9.45％,respectively;the protein-binding rates of naringin were 70.21％ ± 3.31％,67.61％ ± 1.31％,56.00％ ± 3.46％,respectively;the protein-binding rates of gardenioside and rat plasma were 71.56％ ± 3.00 ％,43.56％ ± 12.22％,40.63％ ± 4.73％,respectively;the protein-binding rates ofpaeoniflorin and rat plasma were 55.72％ ± 1.60％,47.59％ ± 6.33％,40.07％ ± 0.72％,respectively;the protein-binding rates of naringin and rat plasma were 85.85％ ± 3.53％,85.38％ ± 0.99％,79.99％ ± 2.57％,respectively;the protein-binding rates of gardenioside and human plasma were 13.56％ ± 2.40％,3.02％ ± 2.41％,5.82％ ± 2.08％,respectively;the protein-binding rates of paeoniflorin and human plasma were 9.49％ ± 5.23％,5.01％ ± 1.10％,3.98％ ± 1.54％,respectively;the protein-binding rates of naringin and human plasma were 25.04％ ± 2.61％,26.09％ ± 13.82％,20.20％ ± 2.24％,respectively.Conclusions Within 5.084,10.04 and 20.08 g/L,the protein binding-rate of gardenioside,paeoniflorin and naringin were shown asfollow,rat plasma ＞bovine serum albumin ＞ human plasma.There were significant differences in species.
