去甲斑蝥素-泊洛沙姆聚合物纳米胶束的制备及表征

摘要

为制备去甲斑蝥素-泊洛沙姆给药系统新制剂,采用薄膜水化法,以泊洛沙姆F127为载体,包载去甲斑蝥素,制备纳米聚合物载药胶束;高效液相色谱法测定胶束的包封率和载药量,动态光散射法测定胶束粒径,紫外分光光度仪以及红外分光光度仪测定并分析药物在胶束中的分布状态. 结果显示去甲斑蝥素被成功包载于聚合物胶束上,聚合物载药胶束的平均粒径为10. 3 nm,粒度分布较窄,包封率和载药量分别约为98%和4 . 67%. 因此,这种载药纳米粒制备工艺简单,粒径小,对疏水性药物有较高的包封率. 这为进一步制备靶向纳米载药胶束打下了基础,为包载其他疏水药物的聚合物胶束的制备提供实验依据.%To prepare a new form of norcantharidin-poloxamer nano-drug delivery systems,a thin-film hy-dration method was used,norcantharidin -loaded polymeric micelles with poloxamer F127 as a novel drug carrier was prepared. Eencapsulation ratio and drug-loading coeffieient,particle size,and drug distribution state of the polymeric micelles were examined by means of HPLC,dynamic light scattering,UV spectrum and FTIR spectrum respectively. The results showed that norcantharidin was successfully entrapped in the polymer micelles with encapsulation ratio of 98%. The drug-loaded polymeric micelles possessed narrow particle size distribution with average particle size of 10. 3 nm,and drug-loading coeffieient of 4. 67%. Therefore,this drug-loaded nanoparticles possess several advantages:simple preparation process, small particle size, and high encapsulation efficiency for hydrophobic drugs. This will set the stage for the further preparation of targe-ted nanoparticles,but also for the preparation of other drug-loaded polymeric micelles offer experimental ba-sis.