小麦生育酚环化酶基因全长cDNA的克隆与分析

摘要

生育酚环化酶(TC)是维生素E生物合成途径中的%Tocopherol cyclase (TC) is a key enzyme for plant vitamin E biosynthesis. The cDNA of wheat TC was cloned by RACE-PCR with primers designed based on a EST (BQ161011) sequence. The full-length cDNA sequence contained a complete open reading frame (ORF) of 1404 bp, which encoding 467 amino acid residues. Homology analysis of the deduced amino acid showed 91%, 88%, 71%, 68% and 67% identity with TC from rice, maize, sesame, potato and Arabidopsis thaliana, respectively. The result of phylogenetic analysis indicated that various TC sequences were clustered into three groups.