野甘草醇对胸腺激酶依赖的更昔洛韦在前列腺癌裸鼠体内的增效作用

摘要

Objective To investigate the synergistic effect of scopadulciol (SDC)on the killing effect on antitumor actions by Ad-hTERT-HSV-tk/GCV suicide gene system on prostate cancer cells in vitro.Methods We successfully built the model of human prostate cancer xenografts in BALB/C nude mouse.The BALB/C nude mice were treated respectively with Ad-hTERT-HSV-tk or GCV in combination with SDC in different doses and different concentrations. The indexes of tumor volume,tumor mass,relative tumor curative effect were evaluated.Results The growth rate of tumor volume of four treatment groups could be significantly inhibited after treatment.The tumor volume and tumor mass of group A,group B,group C and group D were significantly lower than the other groups(P <0.01).The relative tumor curative effect of group A,group B,and group C,which were added SDC,were significantly higher than that of group D (P <0.01).Conclusion The synergistic effect of SDC was significantly shown in treatment of human prostate cancer cells by TK-dependent GCV.%目的：观察野甘草醇（scopadulciol，SDC）在人端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）启动子调控腺病毒介导的单纯疱疹病毒胸苷激酶基因／更昔洛韦（herpes simplex virus thymidine kinase／ganciclovir，HSV-tk／GCV）自杀基因系统在前列腺癌裸鼠体内细胞自杀作用中的增效作用。方法建立前列腺癌裸鼠移植瘤模型，将不同配伍浓度的 SDC、腺病毒－人端粒酶逆转录酶启动子－单纯疱疹病毒－胸苷激酶基因（Ad-hTERT-HSV-tk）、GCV 应用于荷瘤裸鼠，观察肿瘤体积、肿瘤质量、肿瘤抑制率。结果 A、B、C、D 4组肿瘤体积和肿瘤质量均明显小于 E、F、G、H 组，加入 SDC 的 A、B、C 3个治疗组肿瘤体积和肿瘤质量均明显小于未加入 SDC 的 D 组（P ＜0.01）。A、B、C、D 4组肿瘤抑制率明显小于 E、F、G 组，加入 SDC 的 A、B、C 肿瘤抑制率明显小于 D 组（P ＜0.01）。结论通过裸鼠体内实验证实 SDC 对胸腺激酶依赖的 GCV 阻断前列腺癌进展有一定的增效作用。