甲基化修饰对结直肠癌细胞生物学行为的影响及临床应用意义

摘要

Objective To explore the effects and significances of methylated modification on the biological behaviors of colorectal cancer (CRC)cell lines SW620 and LoVo in vitro. Methods SW620 and LoVo were treated with S-Adenosyl-L-methionine (SAM)for six days. Then the flow cytometry assay was used to assessed the changes of cell growth cycle and apoptosis,the transwell assay was used to detect the changs of cell invasion and migration,the plate colony assay was used to testify the changes of cell colony-formation ability,thereby to study the effects of SAM on the biological behaviors of CRC cells.S-Adenosyl-L-homocysteine (SAH)-treated cells and phosphate buffer saline(PBS)-treated cells were used as control groups in the study.Results Compared with either SAH-treated cell groups or PBS-treated ones,the cell cycle of SW620 cell cycle G1 % and LoVo cell cycle S% in SAM group were higher than that in SAH group and PBS group (P < 0.05 ).Compared with SAH- treated cells and PBS- treated ones,the mean cell numbers of SW620 and LoVo treated with SAM were all significantly less than that in SAH group and PBS group through the microporous membrane or the matrigel,the difference is statistically significant(P <0.05).The colony-formation rates of SW620 and LoVo treated with SAM were significantly lesser than that treated with SAH and PBS (P < 0.05 ). Conclusion The exogenous methylation agent can inhibit biological behaviors of CRC cells in vitro.Methylated donor drugs are expected to be effective in the clinical treatment of CRC and provide a preliminary theoretical basis for its clinical application.%目的 研究甲基化修饰对结直肠癌(colorectal cancer,CRC)细胞体外生物学行为的影响及意义.方法 外源性甲基供体药物S-腺苷甲硫氨酸(S-Adenosyl-L-methionine,SAM)处理CRC细胞株SW620和LoVo 6 d后,采用流式细胞术检测细胞生长、凋亡的变化,Tanswell方法观察体外细胞运动迁移能力、趋化侵袭能力的变化,平板克隆形成实验检测细胞克隆增殖能力的变化,从而分析SAM对细胞体外生物学行为的影响.S-腺苷高半胱氨酸(S-Adenosyl-L-homocysteine,SAH)为SAM的同型异构体,SAH组及磷酸盐缓冲液(phosphate buffer saline,PBS)组作为对照.结果 SAM组SW620细胞周期G1%和LoVo细胞周期S%高于SAH组和PBS组(P<0.05).SAM组SW620穿膜细胞数、LoVo穿膜细胞数、SW620穿胶细胞数和LoVo穿胶细胞数明显少于SAH组和PBS组(P<0.05).SAM组SW620细胞克隆形成率和LoVo细胞克隆形成率明显小于SAH组和PBS组(P<0.05).结论 通过外源性甲基修饰能够减弱CRC细胞体外生物学行为能力,甲基供体类药物有望对CRC有疗效,并为其临床应用提供初步理论基础.