Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non- methylated nucleic acids

摘要

The present invention provides a method for detecting a methylated CpG- containing nucleic acid present in a specimen by contacting the specimen with an agent that modifies unmethylated cytosine and amplifying the CpG- containing nucleic acid using CpG-specific oligonucleotide primers. The present invention provides an improved method of methylation detection by facilitating the rapid identification of DNA methylation patterns in a CpG-containing nucleic acid.