鸭新城疫病毒河北分离株血凝素-神经氨酸酶蛋白基因序列分析

摘要

In order to study the genetic variation of Newcastle disease virus (NDV) ,the haem‐agglutinin‐neuraminidase(HN)protein gene of NDV HB/1/05/Dk isolated from Hebei prov‐ince was amplified ,sequenced and genetic analyzed .The full length of H N gene comprised of 2002 nucleotides ,encoding 571 amino acids ,w hich is a typical sequence belonging to velogenic strain .The homology analysis of the nucleotide sequences and amino acids of H N gene indica‐ted the isolate had homologies of 71 .8% to 99 .1% and 83 .4% to 98 .9% with 35 strains . Phylogenetic analysis based on the nucleotide sequences of H N gene showed that the HB/1/05/Dk strain was grouped into the genotype VIId virus ,and had a distant genetic relationship with the vaccine strain e .g .B1 ,LaSota ,Mukteswar ,and strains of ClassⅠ .It is necessary to strengthen the monitoring of waterfowl NDV in Hebei province .%为研究新城疫病毒（Newcastle disease virus ，NDV）的遗传变异特性，对NDV HB／1／05／Dk株的血凝素‐神经氨酸酶（haemagglutinin‐neuraminidase protein ，HN）蛋白基因进行了扩增、测序和遗传进化分析。结果表明：该病毒的 H N基因长2002 bp ，含有1个长为1716 bp的完整开放阅读框架（Open reading frame ，ORF），编码571个氨基酸，符合强毒株的特征。同源性分析表明，该毒株的 H N基因与35株参考毒株间的核苷酸和氨基酸的同源性分别为71．8％～99．1％和83．4％～98．9％。遗传进化分析表明，该毒株与基因 V IId亚型参考毒株关系较近，属同一分支，与疫苗株B1、LaSota、Mukteswar株和I类毒株关系较远。因此，须加强河北省水禽NDV的监测。