研究青龙衣的化学成分及抗肿瘤活性．利用硅胶柱色谱和开放ODS柱色谱等分离技术从青龙衣的95％乙醇提取物中分离得到了11个化合物，通过理化性质、波谱数据分析等方法，鉴定了全部化合物结构．采用MTT法，选取人胃癌细胞株（ SGC7901），人肝癌细胞株（ HepG2），人肺腺癌细胞株（A549），乳腺癌细胞株（MCF7），结肠癌细胞株（LoVo）对化合物体外抗肿瘤作用进行筛选．从青龙衣中分离鉴定了11个化合物，分别为正二十九烷醇（nonacosanol）（1），[1-（4＇-甲氧基苯基）-7-（3＇-甲氧基，2＇-羟基苯基）-3＇，4＇-环氧-3-庚酮[3＇，4＇-epoxy-1-（4＇-methoxylphenyl）-7-（3＇-methoxyl-2＇-hydroxyphenyl）-heptane-3-one]（2），正三十一烷醇（hentriacontanal）（3），1-（4＇-羟基苯基）-7-（3＇-甲氧基-4＇-羟基苯基）-庚烷-3-醇[1-（4＇-hydorxyphenyl）-7-（3＇-me-thoxy-4＇-hydorxyphenyl）-hepta-3-ol]（4），4，6-二羟基-3，4-二氢-1-萘酮（4，6-dihydroxy-3，4-dihydro-naphthalenone）（5），胡桃醌（5-hydroxy-1，4-naphthoquinone）（6），β-谷甾醇（beta-sitosterol）（7），齐墩果酸（oleanolic acid）（8），香草酸（vanillic acid）（9），槲皮素（quercetin）（10），香草醛（vanillin）（11）．化合物5、10对肿瘤细胞均有很强的生长抑制作用，IC50≤35μmol/L．化合物8对肿瘤细胞有较强的生长抑制作用，IC50≤58μmol/L．化合物2、3、6和7对个别肿瘤细胞有作用，其他化合物对肿瘤细胞没有生长抑制作用．11个化合物均为已知化合物，其中化合物1，3，10，11为属内首分，化合物5、8和10均具有较显著的抗肿瘤活性．%To study the chemical constituents in the epicarp of Juglans mandshurica and anti-tumor activity, 11 compounds were isolated from 95%ethanol fraction in the green walnut husks of J .mandshurica by silica gel column chromatography and open ODS column chroma-tography separation techniques .By the method of physicochemical properties and spectral data analysis , all of the compounds structures were identified .MTT assay was used to screen the anti-tumor effect of compounds in vitro , including human gastric cancer cell line ( SGC 7901), human hepatocellular carcinoma cell line (HepG 2), human lung adenocarcinoma cell line ( A 549 ) , breast cancer cell line ( MCF7 ) and colon cancer cell lines ( LoVo ) . Results indicated that Eleven compounds were isolated and identified as nonacosanol (1), [3',4'-epoxy-1-( 4'-methoxylphenyl ) -7-( 3'-methoxyl-2'-hydroxyphenyl ) -hep-tane-3-one](2),hentriacontanal(3),1-(4'-hydorxyphenyl ) -7-(3'-methoxy-4'-hydorxyphenyl)-hepta -3 -ol(4), 4,8 -dihydroxy -3,4 -dihydro -naphthalenone (5), 5-hydroxy -1,4-naphth oquinone(6), beta-sitosterol(7), oleanolic acid(8), vanillic acid(9), quercetin(10), vanillin(11).Compound 5 and 10 had very strong growth inhibition effect on tumor cells with IC 50 less than or equal 35μmol/L.Compound 8 had bet-ter anti-tumor activity than the others with IC 50 less than or equal 58 μmol/L.Compound 2, 3, 6 and 7 had anti-tumor activity on one or two kinds of tumor cells , other compounds had not growth inhibition effect on tumor cells .11 compounds are known compounds , and com-pound 1, 3, 10 and 11 are isolated from Juglans genus for the first time .Compound 5, 8 and 10 have the more significant antineoplastic activity in vitro .
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