铜（II）离子对橙皮素清除自由基作用的影响

摘要

为探讨Cu2＋存在对橙皮素（ Hsp）清除自由基作用的影响．将Hsp与Cu2＋混合即生成不稳定配合物（Hsp－Cu），利用UV－Vis光谱测定Hsp及Hsp－Cu对1，1－二苯基－2－三硝基苯肼自由基（ DPPH·）、羟基自由基（· OH）、超氧阴离子自由基（ O2－·）的清除作用．在实验条件下Hsp－Cu对DPPH·及· OH有一定的清除作用，但清除能力不如Hsp．Hsp－Cu不能有效清除O2－·，且在邻苯三酚自氧化过程中表现出促氧化作用．表明Hsp作为抗氧化剂使用时，应避免与Cu2＋接触，以免影响其抗氧化能力．%To study the affection of Copper ( II ) ions on radical scavenging of hesperetin . Unstable hesperetin -copper ( II ) complex was generated when hesperetin was mixed with copper ( II ) chloride dihydrate .By the contrast tests on the scavenging reaction to DPPH radical, hydroxyl radical (· OH), and superoxide free radical (O2 -· ) of hesperetin -copper ( II) complex and hesperetin , proves that the scavenging activity of DPPH · and · OH of hesperetin-copper ( II) complex was weaker than that of hesperetin .The hesperetin-copper (II) complex can’t scavenge O2 -· showing accelerated oxidation in the process of pyrogallol autoxidation .Hesperetin was used as an antioxidant , cannot coexist with Cu 2+, so as not to affect the antioxidant capacity of hesperetin .