牦牛乳中主要过敏原研究

摘要

为分析牦牛乳中主要的过敏原,腹腔注射牦牛乳蛋白质免疫Balb/C小鼠.未次免疫后,小鼠摘眼球取血,测定特异性抗体和组胺质量浓度.在无菌条件下收集并培养脾淋巴细胞.Western-Blotting方法分析牦牛脱脂乳中主要过敏原组分.结果显示,牦牛脱脂乳中主要的过敏原组分为β-乳球蛋白、α-酪蛋白和β-酪蛋白,这3种过敏原蛋白质致敏强度的大小为β-乳球蛋白〉α-酪蛋白〉β-酪蛋白.因为脾淋巴细胞产生的细胞素IL-4、IL-5质量浓度较高而IFN-γ较低,所以牦牛乳蛋白质为Th2应答.%The aim of this study is to analyze the major allergens in yak milk.The Balb/C mice were sensitized by intraperitoneal injection with yak milk protein.After the last sensitization,blood was collected through the eyes for antibodies and histamine determinations.The lymphocytes derived from spleens were cultured under sterile condition.The major allergens in yak milk were analyzed using western-blotting.The results showed the major allergens in yak milk were β-lactoglobulin,α-casein,and β-casein,and the allergenous extent was β-lactoglobulinα-casenβ-casein.The higher content of IL-4,IL-5 and lower IFN-γ produced by the spleen lymphocyte proliferation indicated that the yak milk protein was the Th2 response.