科罗索酸对妊娠期糖尿病大鼠胎盘组织中基因表达的调节作用及对胰岛β细胞的保护作用研究

摘要

Objective:To study the regulating effect of corosoic acid on gene expression in placental tissue of rats with gestational diabetes mellitus and its protective effect on islet β cells. Methods:SD female rats were divided into corosoric acid group(CA group),gestational diabetes mellitus group(GDM group)and control group,CA group and GDM group were es-tablished into GDM models,and CA group were given corosoric acid intervention. At 20 days of gestation,the expression of insulin signal transducers and inflammatory reaction molecules in placenta and the expression of apoptosis molecules in islet were measured. Results:p-IRS1,p-IRS2,p-PI3K,GLUT1,GLUT3,GLUT4 and visfatin protein expression in placenta tis-sue as well as Bcl-2 mRNA expression in islet tissue of GDM group were significantly lower than those of control group where-as NF-kB,ICAM-1,TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression inislet tissue were significantly higher than those of control group ;p-IRS1 ,p-IRS2 ,p-PI3K ,GLUT1 ,GLUT3 ,GLUT4 and visfatin protein expression in placenta tissue as well as Bcl-2 mRNA expression in islet tissue of CA group were significantly higher than those of GDM group whereas NF-kB ,ICAM-1 ,TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression in islet tissue were significantly lower than those of GDM group. Corosoic acid can improve the insulin resistance and reduce the islet β cell injury in rats with gestational diabetes mellitus.%目的:研究科罗索酸对妊娠期糖尿病大鼠胎盘组织中基因表达的调节作用及对胰岛β细胞的保护作用.方法:SD雌性大鼠分为科罗索酸组(CA组)、妊娠期糖尿病组(GDM 组)和对照组,CA组和GDM 组建立GDM 模型,CA组给予科罗索酸干预.妊娠20天时,测定胎盘中胰岛素信号转导分子、炎症反应分子的表达量以及胰岛中细胞凋亡分子的表达量.结果:GDM 组大鼠胎盘组织中p-IRS1、p-IRS2、p-PI3K、GLUT1、GLUT3、GLUT4、visfatin的蛋白表达量以及胰岛组织中Bcl-2的mRNA 表达量显著低于对照组,NF-kB、ICAM-1、TNF-α、IL-1β的蛋白表达量以及胰岛组织中Bax、Caspase-3的mRNA表达量显著高于对照组;CA 组大鼠胎盘组织中 p-IRS1、p-IRS2、p-PI3K、GLUT1、GLUT3、GLUT4、visfatin的蛋白表达量以及胰岛组织中 Bcl-2的 mRNA 表达量显著高于 GDM 组,NF-kB、ICAM-1、TNF-α、IL-1β的蛋白表达量以及胰岛组织中Bax、Caspase-3的mRNA表达量显著低于GDM组.结论:科罗索酸能够改善妊娠期糖尿病大鼠的胰岛素抵抗并减轻胰岛β细胞的损伤.