Regulating effect of corosoic acid on gene expression in placental tissue of rats with gestational diabetes mellitus and its protective effect on islet β cells

摘要

Objective: To study the regulating effect of corosoic acid on gene expression in placental tissueof rats with gestational diabetes mellitus and its protective effect on islet β cells. Methods:SD female rats were divided into corosoric acid group (CA group), gestational diabetesmellitus group (GDM group) and control group, CA group and GDM group were establishedinto GDM models, and CA group were given corosoric acid intervention. At 20 d of gestation,the expression of insulin signal transducers and inflammatory reaction molecules in placentaand the expression of apoptosis molecules in islet were measured. Results: p-IRS1, p-IRS2,p-PI3K, GLUT1, GLUT3, GLUT4 and visfatin protein expression in placenta tissue as wellas Bcl-2 mRNA expression in islet tissue of GDM group were significantly lower than thoseof control group whereas NF-kB, ICAM-1, TNF-α and IL-1β protein expression in placentatissue as well as Bax and Caspase-3 mRNA expression in islet tissue were significantlyhigher than those of control group; p-IRS1, p-IRS2, p-PI3K, GLUT1, GLUT3, GLUT4 andvisfatin protein expression in placenta tissue as well as Bcl-2 mRNA expression in islet tissueof CA group were significantly higher than those of GDM group whereas NF-kB, ICAM-1,TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNAexpression in islet tissue were significantly lower than those of GDM group. Conclusion:Corosoic acid can improve the insulin resistance and reduce the islet β cell injury in rats withgestational diabetes mellitus.