茶多酚联合表柔比星对膀胱癌细胞凋亡、自噬及浸润性生长的影响

摘要

目的:研究茶多酚联合表柔比星对膀胱癌细胞凋亡、自噬及浸润性生长的影响.方法:培养膀胱癌T24细胞株并分为三组,T P+EPI组用100 μmol/L的茶多酚联合5 μmol/L的表柔比星处理、EPI组用5 μmol/L的表柔比星处理、对照组用不含药物的RPM I培养基处理.处理24小时后,测定细胞中凋亡、自噬及侵袭基因的 mRNA 表达量.结果:TP+ EPI组、EPI组细胞中DAB2IP、PTEN、LC3、Beclin1的mRNA表达量均显著高于对照组,Rce1、YAP、DEK、p62、KPNA2、GRP78、Fra-1、SPOCK1、CX43的mRNA表达量均显著低于对照组;TP+EPI组细胞中DAB2IP、PTEN、p62的mRNA表达量均显著高于EPI组,Rce1、YAP、DEK、LC3、Beclin1、KPNA2、GRP78、Fra-1、SPOCK1、CX43的mRNA表达量均显著低于EPI组.结论:茶多酚联合表柔比星能够促进膀胱癌细胞的凋亡并抑制细胞的自噬及侵袭.%Objective:To study the effect of the tea polyphenol combined with epirubicin on the apoptosis,autophagy and invasive growth of bladder cancer cells.Methods:T24 bladder cancer cell lines were cultured and divided into three groups, TP+EPI group were treated with 100μmol/L tea polyphenol combined with 5μmol/L epirubicin,EPI group were treated with 5μmol/L epirubicin and control group were treated with drug-free RPMI medium.The mRNA expression of apoptosis,autoph-agy and invasion genes was measured after 24 hours of treatment.Results:DAB2IP,PTEN,LC3 and Beclin1 mRNA expres-sion in TP+EPI group and EPI group were significantly higher than those in control group while Rce1,YAP,DEK,p62,KP-NA2,GRP78,Fra-1,SPOCK1 and CX43 mRNA expression were significantly lower than those in control group;DAB2IP, PTEN and p62 mRNA expression in TP+ EPI group were significantly higher than those in EPI group while Rce1,YAP,DEK,LC3,Beclin1,KPNA2,GRP78,Fra-1,SPOCK1 and CX43 mRNA expression were significantly lower than those in EPI group.Conclusion:Tea polyphenol combined with epirubicin can promote the apoptosis and inhibit the autophagy and inva-sion of bladder cancer cells.