脑膜瘤组织中CLDN6基因表达量与MMPs/TIMPs、EMT基因表达量的相关性研究

摘要

Objective:To study the correlation of Claudins6 (CLDN6)gene expression in meningioma tissue with the expressions of matrix metalloproteinases (MMPs)/tissue inhibitors of matrix metalloproteinase (TIMPs)and epithelial-mesen-chymal transition (EMT)genes.Methods:Meningioma tissue samples that were surgically removed in Yibin First People's Hospital between April 2014 and May 2017 were selected,and normal arachnoid tissue samples that were collected from de-compressive craniectomy in this hospital during the same period were selected.The expressions of CLDN6,MMPs/TIMPs and EMT genes in tissues were determined.Results:CLDN6 protein expression in meningioma tissue was significantly lower than that in normal arachnoid tissue;EMMPRIN,MMP2,MMP9,Vimentin and N-cadherin protein expressions in meningioma tissue were significantly higher than those in normal arachnoid tissue while TIMP1,TIMP2,E-cadherin and α-catenin protein expressions were significantly lower than those in normal arachnoid tissue;EMMPRIN,MMP2,MMP9,Vimentin and N-cad-herin protein expressions in meningioma tissue with higher CLDN6 expression were significantly lower than those in meningio-ma tissue with lower CLDN6 expression while TIMP1,TIMP2,E-cadherin andα-catenin protein expressions were significantly higher than those in meningioma tissue with lower CLDN6 expression.Conclusions:Lowly expressed CLDN6 gene in meningio-ma tissue can increase the hydrolysis activity of MMPs,induce epithelial-mesenchymal transition and thus promote the invasive growth of meningioma.%目的:研究脑膜瘤组织中Claudins6(CLDN6)基因表达量与基质金属蛋白酶(MMPs)/基质金属蛋白酶组织抑制因子(TIMPs)、上皮间质转化(EMT)基因表达量的相关性.方法:选择在本院手术切除的脑膜瘤组织样本,同期在宜宾市第一人民医院开颅减压术中收集的正常蛛网膜组织,检测组织中CLDN6、MMPs/TIMPs、EMT基因的表达量.结果:脑膜瘤组织中CLDN6的蛋白表达量显著低于正常蛛网膜组织;脑膜瘤组织中EMMPRIN、MMP2、MMP9、Vimentin、N-cadherin的蛋白表达量均显著高于正常蛛网膜组织,TIMP1、TIMP2、E-cadherin、α-catenin的蛋白表达量均显著低于正常蛛网膜组织;CLDN6高表达脑膜瘤组织中EMMPRIN、MMP2、MMP9、Vimentin、N-cadherin的蛋白表达量显著低于CLDN6低表达脑膜瘤组织,TIMP1、TIMP2、E-cadherin、α-catenin的蛋白表达量显著高于CLDN6低表达脑膜瘤组织.结论:脑膜瘤组织中CLDN6基因的低表达能够引起MMPs水解活性增强、诱导上皮间质转化,进而促进脑膜瘤的浸润性生长.