骨肉瘤组织中抑癌基因TAp73和自噬基因Beclin1的表达及其与肿瘤恶性程度的相关性

摘要

目的:研究骨肉瘤组织中抑癌基因TAp73和自噬基因Beclin1表达量与肿瘤恶性程度的相关性.方法:收集在我院手术切除的56例骨肉瘤组织和肉瘤旁组织、37例骨巨细胞瘤组织,采用免疫组化试剂盒测定TAp73、Beclin1的阳性表达率;采用酶联免疫吸附试剂盒测定TAp73、Beclin1以及增殖、侵袭基因的蛋白表达量.结果:骨肉瘤组织中TAp73、Beclin1的蛋白表达量及阳性表达率均显著低于肉瘤旁组织、骨巨细胞瘤组织,IRX2、JAK3、STAT5、CTGF、Wnt、β-catenin的蛋白表达量均显著高于肉瘤旁组织、骨巨细胞瘤组织;TAp73、Beclin1(+)、(++)、(+++)骨肉瘤组织中IRX2、JAK3、STAT5、CTGF、Wnt、β-catenin的蛋白表达量均显著低于TAp73、Beclin1(-)骨肉瘤组织,TAp73、Beclin1(++)、(+++)骨肉瘤组织中IRX2、JAK3、STAT5、CTGF、Wnt、β-catenin的蛋白表达量均显著低于TAp73、Beclin1(+)骨肉瘤组织,TAp73、Beclin1(+++)骨肉瘤组织中IRX2、JAK3、STAT5、CTGF、Wnt、β-catenin的蛋白表达量均显著低于TAp73、Beclin1(++)骨肉瘤组织.结论:骨肉瘤组织中抑癌基因TAp73和自噬基因Beclin1的低表达能够通过激活IRX2/JAK3/STAT5和CTGF/Wnt/β-catenin信号通路来促进细胞增殖和侵袭.%Objective: To study the correlation of tumor suppressor gene TAp73 and autophagy gene Beclin1 expression in osteosarcoma tissue with tumor malignancy.Methods: A total of 56 cases of osteosarcoma tissues and peri-osteosarcoma tissues as well as 37 cases of giant cell tumor tissues surgically removed in our hospital between June 2013 and August 2016 were collected, immunohistochemical kits were used to detect the positive expression rate of TAp73 and Beclin1, and enzyme-linked immunosorbent assay kits were used to determine the protein expression TAp73, Beclin1 as well as proliferation and invasion genes.Results: Protein expression and positive expression rate of TAp73 and Beclin1 in osteosarcoma tissues were significantly lower than those in peri-osteosarcoma tissues and giant cell tumor tissues, and IRX2, JAK3, STAT5, CTGF, Wnt and β-catenin protein expression were significantly higher than those in peri-osteosarcoma tissues and giant cell tumor tissues;IRX2, JAK3, STAT5, CTGF, Wnt and β-catenin protein expression in osteosarcoma tissues with TAp73 and Beclin1 (+), (++) and (+++) were significantly lower than those in osteosarcoma tissues with TAp73 and Beclin1 (-), IRX2, JAK3, STAT5, CTGF, Wnt and β-catenin protein expression in osteosarcoma tissues with TAp73 and Beclin1 (++) and (+++) were significantly lower than those in osteosarcoma tissues with TAp73 and Beclin1 (+), and IRX2, JAK3, STAT5, CTGF, Wnt and β-catenin protein expression in osteosarcoma tissues with TAp73 and Beclin1 (+++) were significantly lower than those in osteosarcoma tissues with TAp73 and Beclin1 (++).Conclusions: Lowly expressed tumor suppressor gene TAp73 and autophagy gene Beclin1 in osteosarcoma tissue can activate IRX2/JAK3/STAT5 and CTGF/Wnt/β-catenin signaling pathways to promote cell proliferation and invasion.