mRNA and protein expression of autophagy marker molecule Beclin1 in osteosarcoma tissue and their correlation with pathological features

摘要

Objective: To discuss the mRNA and protein expression of autophagy marker molecule Beclin1 in osteosarcoma tissue and their correlation with pathological features. Methods: A total of 60 patients with osteosarcoma receiving surgical treatment in the hospital between April 2012 and January 2016 were selected, and the osteosarcoma tissue and adjacent normal tissue samples were collected during operation. Fluorescence quantitative PCR method was used to detect the expression of Beclin1 mRNA as well as proliferation and invasion gene expression in the tissue specimens, and the Beclin1 protein expression was detected by western-blot method. According to the median of Beclin1 mRNA and protein expression in osteosarcoma tissues, they were further grouped into high Beclin1 mRNA expression group (n=30) and low Beclin1 mRNA expression group (n=30) as well as high Beclin1 protein expression group (n=30) and low Beclin1 protein expression group (n=30). The differences in proliferation and invasion gene expression in osteosarcoma tissue with different Beclin1 mRNA and Beclin1 protein expression were compared. Results: Beclin1 mRNA and Beclin1 protein expression in osteosarcoma tissue were lower than those in adjacent normal tissue;proliferation gene KISS-1 mRNA expression in low Beclin1 mRNA expression group of osteosarcoma tissue was lower than that in high Beclin1 mRNA expression group while Six1, FoxM1, RIPK4 and STIM1 mRNA expression were higher than those in high Beclin1 mRNA expression group; invasion gene DLX1 mRNA expression was lower than that in high Beclin1 mRNA expression group while EFEMP1, MTA1, Notch1, HES1 and LEF-1 mRNA expression were higher than those in high Beclin1 mRNA expression group; proliferation gene KISS-1 mRNA expression in low Beclin1 protein expression group of osteosarcoma tissue was lower than that in high Beclin1 protein expression group while Six1, FoxM1, RIPK4 and STIM1 mRNA expression were higher than those in high Beclin1 protein expression group; invasion gene DLX1 mRNA expression was lower than that in high Beclin1 protein expression group while EFEMP1, MTA1, Notch1, HES1 and LEF-1 mRNA expression were higher than those in high Beclin1 protein expression group. Conclusion: Both mRNA and protein expression of autophagy marker molecule Beclin1 decrease in osteosarcoma tissue and are directly correlated with the proliferation and invasion activity of tumor cells.