糖尿病合并牙周炎患者龈沟液中OPG/RANKL表达与炎症因子、自由基生成及骨代谢的相关性

摘要

目的:研究糖尿病合并牙周炎患者龈沟液中 OPG/RANKL 表达与炎症因子、自由基生成及骨代谢的相关性.方法:选择在西安市第四医院就诊的糖尿病合并牙周炎患者作为研究的牙周炎组,选择同期接受牙周检查的健康志愿者作为对照组.采集龈沟液并测定OPG/RANKL的表达量、炎症因子及骨代谢分子的含量、自由基的生成量.结果:牙周炎组龈沟液中 OPG 的蛋白表达量及 OPG/RANKL表达量的比值、ICTP、Runx2的含量均显著低于对照组,RANKL 的蛋白表达量、TNF-α、IL-1β、IL-17、HMGB1、DKK1、MMP1的含量及ROS、MDA、8-OHdG的生成量均显著高于对照组;牙周炎组患者龈沟液中OPG/RANKL表达量的比值与TNF-α、IL-1β、IL-17、HMGB1、DKK1、MMP1的含量及ROS、MDA、8-OHdG的生成量呈负相关关系,与ICTP、Runx2的含量呈正相关关系.结论:糖尿病合并牙周炎患者牙周组织中炎症及氧化应激反应的过度激活能够引起OPG/RANKL表达紊乱,进而造成骨代谢异常.%Objective:To study the correlation of OPG/RANKL expression in gingival crevicular fluid with inflammato-ry factors,free radical generation and bone metabolism in patients with diabetes mellitus complicated by periodontitis.Meth-ods:Patients with diabetes mellitus complicated by periodontitis who were treated in Xi 'an fourth hospital between March 2015 and May 2017 were selected as the periodontitis group for the research,and healthy volunteers who underwent periodontal ex-amination during the same period were selected as the control group.The gingival crevicular fluid was collected to determine the expression of OPG/RANKL,the contents of inflammatory factors and bone metabolism molecules as well as the generation of free radicals.Results:OPG protein expression,OPG/RANKL expression ratio as well as ICTP and Runx2 contents in gingival crevicular fluid of periodontitis group were significantly lower than those of control group whereas RANKL protein expression, TNF-α,IL-1β,IL-17,HMGB1,DKK1 and MMP1 contents as well as ROS,MDA and 8-OHdG generation were significantly higher than those of control group;OPG/RANKL expression ratio in gingival crevicular fluid of periodontitis group was nega-tively correlated with TNF-α,IL-1β,IL-17,HMGB1,DKK1 and MMP1 contents as well as ROS,MDA and 8-OHdG genera-tion,and positively correlated with ICTP and Runx2 contents.Conclusion:The excessive activation of inflammation and oxida-tive stress in periodontal tissue of patients with diabetes mellitus complicated by periodontitis can cause OPG /RANKL expres-sion disorder and then lead to bone metabolism disorder.