硫辛酸辅助治疗对糖尿病足患者全身氧化应激及局部炎症反应的影响

摘要

Objective:To study the effect of adjuvant lipoic acid therapy on the systemic oxidative stress response and local inflammatory response in patients with diabetic foot.Methods:Patients with diabetic foot who received conservative treat-ment in Qinzhou Traditional Chinese and Western Medicine Hospital of Guangxi Province between June 2014 and April 2017 were selected as the research subjects and randomly divided into the lipoic acid group who received lipoic acid combined with conventional therapy and the control group who received conventional therapy.The contents of oxidative stress indexes in serum were measured before treatment and 1 week after treatment;the contents of inflammatory response indexes and apoptosis inde-xes in wound tissue were determined 1 week after treatment.Results:1 week after treatment,serum SOD,GPx and CAT con-tents of both groups of patients were higher than those before treatment(P<0.05)whereas MDA and AOPP contents were significantly lower than those before treatment(P<0.05),and serum SOD,GPx and CAT contents of lipoic acid group were significantly higher than those of control group whereas MDA and AOPP contents were significantly lower than those control group(P<0.05);1 week after treatment,TLR2,TLR9,ICAM1,IL-1,IL-6,TNF-α,AIF,Bax,Fas,FasL and Caspase-3 contents in the wound of lipoic acid group were significantly lower than those of control group(P<0.05)whereas Bcl-2 content was significantly higher than that of control group(P<0.05).Conclusion:Adjuvant lipoic acid treatment of diabetic foot can reduce the systemic oxidative stress response and local inflammatory response.%目的:研究硫辛酸辅助治疗对糖尿病足患者全身氧化应激及局部炎症反应的影响.方法:选择2014年6月~2017年4月在广西钦州市中西医结合医院接受保守治疗的糖尿病足患者作为研究对象,随机分为接受硫辛酸联合常规治疗的硫辛酸组、接受常规治疗的对照组.治疗前及治疗后1周时,测定血清中氧化应激指标的含量;治疗后1周时,测定创面组织中炎症反应指标、细胞凋亡指标的含量.结果:治疗后1周时,两组患者血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、CA T 的含量均高于治疗前,丙二醛(M DA)、晚期氧化蛋白产物(A O PP)含量均显著低于治疗前,且硫辛酸组血清中SOD、GPx、CAT的含量显著高于对照组(P<0.05),MDA、AOPP的含量均显著低于对照组(P<0.05);治疗后1周时,硫辛酸组患者创面内 TLR2、TLR9、细胞间黏附分子(ICAM)1、白介素(IL)-1、IL-6、肿瘤坏死因子-α(TNF-α)、AIF、Bax、Fas、FasL、Caspase-3的含量均显著低于对照组(P<0.05), Bcl-2的含量显著高于对照组(P<0.05).结论:硫辛酸辅助治疗糖尿病足能够减轻全身氧化应激反应及局部炎症反应.