小鼠肠缺血再灌注诱发多器官功能障碍的动物模型研究

摘要

Objective:To establish an animal model of multiple organ dysfunciton syndrome (MODS)induced by intestinal ischemia /reperfusion (II /R)in mice.Methods:C57 BL/6 mice were randomly divided into group 1(intestinal ischemia 30,40,50 and 60 min subgroups)and group 2(sham operation subgroup ,II /R subgroup).The survival rates of mice were observed after different intestinal ischemia time in group 1.Biochemical indexes of ALT,AST,Crea and LDH in serum were measured,and pathological changes of the lung,liver and kidney were observed by optical microscope after II /R in group 2.Results:With intestinal ischemia time prolonged,mice survival rate was signifi-cantly reduced.Compared with intestinal ischemia 30 min group,the mice survival rates in more than 50 min intestinal ischemia group decreased significantly (P <0.05).The mice survival rates in intes-tinal ischemia 60 min group decreased significantly than in less than 50 min intestinal ischemia group (P <0.05).After intestinal ischemia 40 min and reperfusion 6 h,mice serum ALT,AST,Crea and LDH increased significantly (P <0.05)and pathological changes of lung,liver and kidney could be observed.Conclusion:6 h of reperfusion after mesentery ischemia for 40 ~50 min is the ideal choice to research MODS induced by II /R in mice.%目的：探讨建立稳定的肠缺血再灌注（II ／R）损伤诱发多器官功能障碍综合征（MODS）的实验动物模型。方法：C57 BL／6小鼠72只，根据观察指标随机分组；分组1，肠缺血30 min 组、40 min 组、50 min 组和60 min组后恢复灌注，观察造模后7 d 不同肠缺血时间动物生存率；分组2，假手术组（Sham）、缺血40 min 后再灌注组（II ／R），于再灌注1 h、6 h 及12 h 取标本，检测血清 ALT、AST、Crea 及 LDH 水平，同时取肺、肝及肾组织做病理学检查。结果：随着肠缺血时间延长，动物生存率明显降低，肠缺血40 min 小鼠7 d 内生存率为60％，选择缺血40 min作为动物模型进行分组2实验；肠缺血40 min 后再灌注6 h 时，血清 ALT、AST、Crea 和 LDH 明显升高（P ＜0．05），肺、肝脏及肾脏组织器官发生病理学改变。结论：小鼠肠缺血40 min 再灌注后6 h，是研究 II ／R 损伤诱发 MODS 较理想的时间点。