目的：探讨地黄饮子对急性脑缺血大鼠的学习记忆能力影响。方法：选取Wistar大鼠72只随机均分为实验1组、实验2组、模型组和正常对照组，前3组大鼠复制急性脑缺血模型，正常对照组大鼠手术但不给予脑缺血；实验1组造模后每日灌服地黄饮子汤36 g/kg，实验2组造模后每日灌服尼莫地平12.5 mg/kg，模型组和正常对照组造模后1次/d盐水灌胃，各组大鼠连续灌胃3周；所有大鼠进行Morris水迷宫定位航行实验记录逃避潜伏期测试学习能力，跳台实验记录逃避潜伏期评价记忆能力，并用电镜图像分析系统计算4组大鼠脑梗死面积。结果：与正常对照组比较，模型组大鼠定位航行实验潜伏期明显延长，差异有统计学意义（P <0.05）；与模型组比较，实验1组和实验2组大鼠的定位航行实验潜伏期明显缩短，差异有统计学意义（ P <0.05）；与正常对照组比较，模型组大鼠跳台实验潜伏期明显延长，差异有统计学意义（ P<0.05）；与模型组比较，实验1组和实验2组大鼠的跳台实验潜伏期明显缩短，差异有统计学意义（ P<0.05）；实验1组脑梗死面积明显小于模型组和实验2组，差异有统计学意义（ P<0.05）。结论：地黄饮子可以提高脑缺血再灌注大鼠的学习能力和记忆能力，减少缺血对脑组织神经元的损伤。%Objective:To explore the effect of Rehmannia YinZi on learning and memory ability of a-cute cerebral ischemia rats. Methods:72 Wistar rats were randomly divided into experimental group 1,experimental group 2,model group and normal control group. The acute cerebral ischemia model was established in the first 3 groups of rats while the normal control group did not received cerebral is-chemia surgery. Experiment 1group received daily intragastric administration of Dihuangyinzi Decoction (36 g/kg)after modeling,experimental group 2 received daily intragastric administration of nimodip-ine(12. 5 mg/kg)after modeling,and model group and normal control group received 1/d saline after modeling. All rats were administered orally for 3 consecutive weeks. All rats were subjected to Morris water maze navigation experiment to for memory capacity evaluation. Electron microscope image analy-sis system was adopted to calculate cerebral infarction area for the 4 groups of rats. Results:Compared with normal control group,the experimental latency of model group was significantly prolonged,and the difference was statistically significant(P<0. 05). Compared with model group,the experimental latency in experimental group 1 and experimental 2 were significantly shortened,the difference was sta-tistically significant(P<0. 05). Compared with normal control group,the step-down test latency of rats in model group was significantly increased,and the difference was statistically significant( P <0. 05). Compared with model group,the step-down test latency of experimental group 1 and experi-mental group 2 rats were significantly shorter,and the difference was statistically significant( P <0. 05 );The cerebral infarction area in experimental group1 was significantly smaller than the that of model group and experimental group 2 ,and the difference was statistically significant( P <0 . 05 ). Conclusion:Rehmannia YinZi can improve learning and memory ability of cerebral ischemia reperfu-sion rats,and reduce the damage of brain neurons resulted from ischemia.
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