[目的] 观察中药复方凉血化瘀方对自发性系统性红斑狼疮 (SLE) 模型MRL/lpr小鼠抗双链DNA抗体 (dsDNA)、 抗核小体抗体 (AnuA) 及血清白细胞介素6 (IL-6)、 IL-17、 IL-21表达的影响.[方法] 实验小鼠分为空白对照组、 模型组、中药高剂量组 (剂量为25.2 g/kg)、 中药低剂量组 (剂量为12.6 g/kg), 连续给药8周, 眼眶取血并分离血清, 采用酶联免疫吸附 (ELISA) 法检测抗dsDNA抗体、 AnuA、 IL-6、 IL-17、 IL-21水平. [结果] 模型组小鼠血清自身抗体和IL-6、 IL-17、IL-21水平较空白对照组显著升高 (P<0.01), 中药组血清抗体和细胞因子水平较模型组降低, 其中中药高剂量组与模型组比较, 差异均有统计学意义 (P<0.01). [结论] 凉血化瘀方能降低自身抗体水平, 抑制炎性细胞因子的表达, 是其有效治疗SLE的机制之一.%Objective To observe the effect of blood-cooling and blood-stasis-removing Decoction on anti-double-strain DNA ( anti-dsDNA) antibody, anti-nucleosome antibody ( AnuA) and serum levels of interleukin ( IL) -6, 17, 21 in spontaneous systemic lupus erythematosus ( SLE) MRL/lpr experimental rats. Methods The experimental rats were divided into blank control group, model group, and high- and low- dose Chinese medicine groups ( 25.2, 12.6 g/kg respectively) , the treatment lasting 8 weeks. At the end of the experiment, the blood taken from the orbital veins was separated for obtaining serum, and then the serum anti-dsDNA antibody, AnuA, IL-6,17,21 levels were tested by enzyme-linked immunosorbent assay ( ELISA). Results The serum autoantibody and IL-6, 17, 21 levels of rats in the model group were increased significantly as compared with the blank control group ( P<0.01). The serum antibody and cytokine levels of Chinese medicine groups were reduced as compared with the model group, the difference between high-dose Chinese medicine group and model group being significant ( P<0.01). Conclusion Blood-cooling and blood-stasis-removing Decoction has certain effect on reducing the serum levels of anti-dsDNA antibody, AnuA, and IL-6,17,21, which may coniribute to one of its therapeutic mechanisms for SLE.
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