siRNA干扰内源性TM9SF1基因对人脐静脉内皮细胞炎症因子及血管紧张素转化酶1表达的影响

摘要

目的:探讨siRNA干扰内源性TM9SF1基因对人脐静脉内皮细胞(HUVEC)炎症因子及血管紧张素转化酶1(ACE1)表达的影响.方法:将HUVEC分为阴性对照组(si-NC组)和转染组(si-TM9SF1组).以脂质体Lipofectamine 3000将TM9SF1基因特异性siRNA转染至HUVEC细胞,采用实时荧光定量PCR (qPCR)方法检测特异性siRNA对TM9SF1基因的干扰效果,并比较两组白介素(IL)-1β、IL-8和ACE1基因的相对表达量.结果:转染TM9SF1基因特异性siRNA 48 h后,si-TM9SF1组细胞中TM9SF1基因相对表达量明显低于si-NC组(P＜0.05),且si-TM9SF1组IL-1β、IL-8和ACE1基因相对表达量亦明显低于si-NC组(P＜0.05或P＜0.001).结论:干扰内源性TM9 SF1可明显抑制HUVEC中炎症因子IL-1β、IL-8及血管收缩相关基因ACE1的表达.%Objective:To study the effects of the interference of siRNA with endogenous TM9SF1 gene on the inflammation-related genes and angiotensin converting enzyme 1 (ACE1) in human umbilical vein endothelial cells (HUVEC).Methods:The experiment was divided into a negative control group (si-NC group) and a interference group (si-TM9SF1 group).The specific siRNA of TM9SF1 was synthesized and transfected into HUVEC.The mRNA expressions of interleukin (IL)-1β,IL8 and ACE1 were detected by real-time fluorescence quantitative PCR (qPCR).Results:The mRNA expression of TM9SF1 in si-TM9SF1 group was significantly lower than that in si-NC group 48 h after the transfection (P＜0.05).The mRNA expressions of IL-1β,IL-8 and ACE1 in si-TM9SF1 group were down-regulated,compared to si-NC group (P＜0.05 or P＜0.001).Conclusion:Interference of siRNA with endogenous TM9SF1 could significantly inhibit the expression of inflammation related genes (IL-1β,IL-8) and ACE1 in HUVEC.