苯甲酸降解途径及转化生产粘康酸的研究进展

摘要

苯甲酸是多种芳香化合物生物代谢的重要中间物，对微生物降解苯甲酸的生物化学和遗传学研究，有利于阐明芳香化合物的生物降解机制，分离和培育出降解谱广和降解性能高的菌株应用于废水处理、环境修复和生物转化。苯甲酸的儿茶酚邻位裂解（ortho-cleavage）途径会产生粘康酸，后者是一种潜在的平台化合物，用于生产新型功能树脂、生物塑料、食品添加剂等产品。本文综述了微生物降解苯甲酸和转化生产粘康酸的研究和进展，并提出该领域研究今后的发展方向，为后续研究提供有价值的参考。%Benzoate is a model aromatic compound,which is also an important intermediate of a variety of aromatic compounds. Studying microbial degradation of benzoate provides knowledge on the metabolic mechanism of other aromatic compounds,facilitates the screen of efficient bacteria for wastewater treatment,environmental remediation and biotransforma-tion.Benzoate can be metabolized via catechol by means of the ortho-cleavage pathway to yield cis,cis-muconic acid,which is suggested to be an interesting platform chemical for the pro-duction of new functional resins,bio-plastics and food additives.In this paper,various benzoate degradation pathways and cis,cis-muconic acid production by biotransformation of benzoate are reviewed, and guidelines for developing high performance microbial cell factory for cis,cis-muconic acid production are also proposed.