荧光光谱法研究绞股蓝皂苷与人血清白蛋白的相互作用

摘要

在pH=7.40的Tris-HCl缓冲溶液中,用荧光光谱法研究了绞股蓝皂苷与人血清白蛋白（HSA）相互作用的情况.结果表明,绞股蓝皂苷对人血清白蛋白的内源荧光有明显的猝灭作用,猝灭过程为动态猝灭.并计算得到绞股蓝皂苷与HSA的结合位点数和结合常数.通过热力学数据分析,推断出绞股蓝皂苷与人血清白蛋白之间主要靠疏水作用结合.同时采用同步荧光技术考察了绞股蓝皂苷对HSA构象的影响.%The interaction between stevenleaf and human serum albumin(HSA) in buffer solution(pH=7.40) were studied by fluorescence spectroscopy.It was found that Stevenleaf quenched the fluorescence of HSA via a dynamic quenching process.The binding constant and number of binding sites were found.Values of thermodynamic parameters were calculated.These dates indicated that hydrophobic played a major role in the binding of Stevenleaf and HSA.Synchronous fluorescence spectroscopy was used in the study of the effect of Stevenleaf on the configuration of HSA.