Elimination of Scatter Background in Synchronous Excitation Spectrofluorometry by the Use of Phase-Resolved Fluorescence Spectroscopy

摘要

Phase-resolved fluorescence spectroscopy was used to suppress the signal due to scattered light in synchronous excitation spectra. The spectra were collected by synchronously scanning the emission and excitation monochromators with a constant wavelength difference of 3 nm maintained between them. Detection limits for five polycyclic aromatic hydrocarbons were better than or similar to those obtained by using steady-state synchronous excitation.