采用冷冻干燥法制备了丝素蛋白(SF)/纳米生物玻璃(NBG)复合多孔支架材料.并用XRD、FT-IR、SEM等对SF/NBG复合支架进行了结构与性能表征.结果表明,SF/NBG复合多孔支架孔连通性较好,孔径为150～300μm,孔隙率为80.6％～90.3％；同时NBG的加入促进了复合多孔支架中SF的构象部分由无规卷曲向β-折叠转变.复合多孔支架抗压强度和抗压模量相比于纯SF多孔支架有较大提高.采用模拟体液浸泡实验研究了复合支架的体外生物活性,并用XRD、FT-IR和FESEM对试样表面进行了表征.结果显示,复合多孔支架经模拟体液浸泡7d后,表面沉积出类骨羟基磷灰石(HA)层,NBG的加入能加快复合多孔支架表面沉积类骨HA的速度.研究结果显示SF/NBG复合多孔支架材料有望作为生物活性良好的骨组织修复材料.%In this paper, the silk fibroinano-bioactive glass(SF/NBG) porous composite scaffolds were pre-pared by freezing-drying and characterized by X-ray diffraction(XRD), fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy(SEM). The results showed that SF/NBG composite scaffolds had better interconnected porous structures, and the pore size was about 150 to 300μm, the porosity was between 80. 6% and 90. 3%. Simultaneously, the conformation of silk fibroin was promoted to change from random coil to β-sheet structure with NBG added into it. Porous composite scaffolds had higher compressive strength and compressive modulus compared to pure SF porous scaffold. The bioactivity in vitro of composite scaffolds were studied through immersing it into simulated body fluid, the samples were also characterized by XRD, FT-IR and FESEM. The results indicated that hydroxyapatite(HA) layers were formed on the porous composite scaf-folds after immersed in SBF for seven days, the speed of HA deposition was accelerated with NBG adding into SF. The result was that SF/NBG porous composite scaffolds were expected to be as good bioactivity materials for bone tissue repairing.
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