碘化钠和IL-1β对人甲状腺上皮细胞TRAIL表达的影响

摘要

Objective To investigate the effects of sodium iodide and IL-1β on the expression of TNF-related apoptosis-inducing ligand (TRAIL) and apoptosis of human thyrocytes primary cultured in vitro. Methods Human thyroid epithelial cells were primary cultured from normal thyroid tissues, or tissues -with Graves Disease(GD) and Hashimoto thyroiditis (HT). They -were cultured in the absence or presence of sodium iodide or IL-1β -with different concentrations, -which -were separated into normal control, HT and GD groups. Expressions and distributions of TRAIL in thyrocytes of HT, GD and normal groups were investigated with immunocytochemistry method and flow cytometry was used to determine theexpression of TRAIL and apoptosis. Results (1) The expression level of TRAIL in GD and HT thyrocytes -was increased significantly compared to control group(P＜0. 05). (2) The positive rate of TRAIL in the normal and GD group was significantly higher with 10 mg/L sodium iodide than that with 1 mg/L sodium iodide (P＜0. 05). In the HT group, the expression of TRAIL with 100 mg/L sodium iodide was significantly higher than that -with 1 mg/L sodium iodide(P＜0. 01). (3)The rate of apoptosis in the human thyrocytes from HT group -was significantly higher than that from the normal or GD group (P ＜0. 01). (4) A high concentration of cytokine IL-1β could induce TRAIL expression in all three groups of thyrocytes. Conclusions The expression of TRAIL in the human thyrocytes from thyroid tissues with GD or HT is higher than those from normal tissues. Under the condition of excess iodine or IL-1β, the expression of TRAIL is increased, -which may induced apoptosis of thyroid cells, and further lead to functional and pathologic changes in thyroid grand.%目的 研究不同浓度碘化钠(NaI)和白细胞介素-1β(IL-1β)对体外培养的原代人甲状腺上皮细胞(TEC)肿瘤坏死因子相关凋亡诱导配体(TRAIL)的表达及凋亡的影响,探讨自身免疫性甲状腺疾病(AITD)的可能发病机制.方法 收集正常、Graves病(GD)和桥本甲状腺炎(HT)患者甲状腺组织进行原代细胞培养,以不同浓度NaI和IL-1β刺激单层培养的TEC,免疫细胞化学方法检测TRAIL表达和分布情况,流式细胞术检测干预后细胞TRAIL阳性表达率及细胞凋亡率的变化.结果 (1)GD组和HT组TEC的TRAIL阳性百分率与正常组比较显著增加(P<0.05).(2)正常组及GD组在10 mg/L NaI干预下与1 mg/L浓度组比较,TRAIL阳性率明显升高(P<0.05);HT组在100 mg/L NaI干预下与1 mg/L NaI浓度组比较,TRAIL表达明显增加(P<0.01).(3)HT组的TEC凋亡率高于正常组和GD组(P<0.01).(4)高浓度的IL-1β对TRAIL表达有上调作用.结论 AITD患者TEC的TRAIL表达增强,且高碘及IL-1β可上调TRAIL表达强度,使凋亡增加,进而可能影响其功能及病理生理改变.