目的采用腹部切口加结肠注射辣椒素以及气囊扩张建立大鼠结肠手术术后疼痛动物模型。方法SD大鼠24只，随机分为4组（n＝6）：假手术组（A组）、单纯腹部切口手术组（B组）、腹部切口加辣椒素结肠注射组（C组）及腹部切口加辣椒素结肠注射并球囊扩张结肠组（D组）。观察大鼠腹部切口前及腹部切口后6，24，30，48 h时间点的30 min内脏痛积分，测定左后足底热痛阈及腹部切口周围皮肤的机械痛阈。实验结束后大鼠结肠行病理切片观察。结果与基础值比较，大鼠腹部切口后切口周围皮肤机械痛阈下降，并且持续到术后48 h以后（P＜0．01）；与A组比较，C组和D组表现出明显的内脏痛反应，内脏痛积分显著增高（P＜0．01）；与基础值比较，A组和B组足部热痛阈无明显变化；与A组比较，C组和D组足部的热痛阈在结肠刺激后升高（P＜0．05）；C组和D组结肠组织黏膜下层可见水肿。结论腹部切口加气囊扩张并注射辣椒素结肠可建立较好的结肠术后疼痛模型。%Objective To develop a model of post-operative pain after colon surgery through abdominal incision combined with colon administration of capsaicin and balloon distension . Methods 24 healthy adult Sprague-Dawley rats were assigned to four groups :group A ,received anesthesia only ;group B ,anesthesia with abdominal incision ;group C ,abdominal incision plus capsaicin colon administra-tion ;group D ,abdominal incision plus colon administration of capsaicin and balloon distension . Visceral pain-related behavior changes ,and withdrawal latencies to noxious mechanical and thermal challenges were used to assess pain behavior . Morphological changes of rat colon tissue cells were observed as well . Results Compared with baseline ,pain threshold around abdomen in rats decreased after abdominal inci-sion and lasted over 48 h(P<0 .01) . Rats in group C and group D had obvious visceral pain reaction and pain scores increased significantly especially in group D (P<0 .01) . Compared with baseline ,paw with-draw thermal latency in group A and group B had no obvious change ;Compared with Group A ,paw with-draw thermal latency in group C and group D increased significantly after colon stimulation (P<0 .05) . Edema in colon tissue was observed in group C and group D . Conclusion Abdominal incision combined with colon administration of capsaicin and balloon distension presents a new simple reproducible rat model of postoperative pain model after colonic surgery .
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