5-氨基酮戊酸光动力作用对白念珠菌超微结构的影响

摘要

目的：观察5‐氨基酮戊酸光动力对白念珠菌细胞超微结构的影响，为研究5‐氨基酮戊酸光动力抗菌的作用机制提供理论基础。方法设置实验组（5‐氨基酮戊酸光动力作用后的白念珠菌组）及对照组，分别在透射电镜下观察2组菌株细胞的超微结构。结果透射电镜下，5‐氨基酮戊酸光动力作用后的白念珠菌细胞肿胀变形，细胞壁、细胞膜结构不完整，细胞壁疏松，细胞膜与细胞壁之间间隙大小不一，部分细胞可见细胞壁棉絮状致密外层或细胞膜局灶性缺失的现象。结论5‐氨基酮戊酸光动力对白念珠菌的细胞壁结构具有一定的影响。%Objective To observe the influence of 5‐aminolevulinic acid photodynamic on ultra‐structure of Candida albicans and to provide a theoretical basis forstudyingantifungal mechanism of ALA‐PDT . Methods Ultrastructure of Candida albicans in experimental group(subjected to 5‐aminolevulinic acid photodynamic)and in control group were observed under transmission electron microscope . Results Under transmission electron microscope ,the cells ,after being subjected to 5‐aminolevulinic acid photody‐namic ,presentedswelling deformation ,showing incomplete walland membrane structure . The cell wall was loose ,and the gap between the cell membrane and cell wall varied in sizes . We also observed ,in some cells ,incomplete cell wall structure with electron dense layers and cell membrane discontinuation at localized area . Conclusions 5‐aminolevulinic acid photodynamic may play a role in the structure of cell w all of Candida albicans .