柑橘黄龙病碘—淀粉法快速检测技术优化

摘要

To improve citrus huanglongbing detection method for rapid field test, iodo-starch reaction technology was optimized in terms of frozen time and rubbing leaf epidermis. The results showed that rubbing leaf epidermis was an alternative to frozen treatment without compromising diagnosis accuracy, achieving 92.5% consistency with PCR. So that citrus leaf with rubbed epidermis can be de-colorized directly. Averagely, decoloration time was reduced by 7.17-8.47 h, with about 5.0 h saved in winter and 3.7 h in other seasons.%为使一种柑橘黄龙病碘—淀粉法快速检测技术更好地应用于大田检测,以砂糖橘为试验材料,研究无需冷冻和缩短叶片脱色时间的技术.结果表明,磨掉叶片正反面表面蜡质层后,直接脱色,可以省去冷冻处理步骤,且不影响检测结果;检测结果与常规PCR检出结果的一致率为92.5%;同时,叶片脱色时间缩短7.17~8.47 h.优化后,冬季样品叶绿素脱除所需时间均值为5.0 h,其他季节叶片脱色时间均值为3.7 h.