目的 探讨成纤维细胞激活蛋白α(fibroblasts activating protein α,FAPα)和转化生长因子β1 (trans-forming growth factor-β1,TGF-β1)在急性心肌缺血猝死者心肌细胞胞浆中的表达. 方法 选取47例尸检案例心脏标本,按照死因分为对照组、急性心肌梗死组及复发性心肌梗死组,应用免疫组织化学技术检测心肌细胞胞浆中FAPα和TGF-β1的表达,染色结果经图像分析系统采集照片后,测定其阳性面积率及平均光密度值,比较各组间阳性信号的差别.结果 急性心肌梗死组和复发性心肌梗死组的心肌缺血区心肌细胞胞浆中FAPα和TGF-β1呈强阳性表达,对照组心肌细胞胞浆中FAPα无表达,TGF-β1呈弱阳性表达.急性心肌梗死组和复发性心肌梗死组分别与对照组心肌细胞内FAPα和TGF-β1相比,差异有统计学意义(P＜0.05). 结论 FAPα和TGF-β1联合应用可作为急性心肌缺血猝死的诊断指标.%Objective To explore the expression of fibroblast activation protein a (FAPα) and transforming growth factor-β1 (TGF-β1) in myocardial cytoplasm for the cases of sudden death due to acute myocar-dial ischemia. Methods The heart tissues of 47 cases were collected. All cases were divided into three groups: control group, acute myocardial infarction group and recurrent myocardial infarction group. FAPa and TGF-β1 expressions were explored in myocardial cytoplasm by immunohistochemistry technology. The staining results were collected by image analysis system and then the positive area ratio and average optical density were detected. The positive signal differences were compared among the groups. Results Strong FAPa and TGF-β1 expressions were detected in myocardial cytoplasm in both acute and recurrent myocardial infarction groups. The expression of FAPa was not detected in myocardial cytoplasm in control group and TGF-β1 expression showed a weak positive result. FAPa and TGF-β1 expressions showed the statistical difference (P<0.05) in myocardial infarction (acute and recurrent) groups and control group. Conclusion FAPa and TGF-β1 can be the diagnostic markers for determing acute myocardial infarction.
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