isolated nucleic acid molecule, expression vector, isolated recombinant cell, transgenic plant or part of plant, isolated protein, isolated antibody, method for determining if a plant possesses citrus sudden death virus, method for determining the presence of citrus sudden death in a host organism and method for determining the presence of citrus sudden death virus in a plant population

摘要

"NUCLEIC ACID MOLECULA ISOLATED, EXPRESSION VECTOR, ISOLATED RECOMBINANT CELL, TRANSGENIC PLANT OR PART, ISOLATED PROTEIN, ISOLATED ANTIBODY, METHOD OF DETERMINING IF A SITE OF THE VITUS OF THE SITE OF THE VITUES Of the sudden death of citrus in a host organism and method for determining the presence of the virus of the sudden death of citrus in a population of plants ". The present invention relates to nucleic acid molecules comprising the Citrus Sudden Death Virus (CSDV) genome, the causative agent of Citrus Sudden Death disease. Cloned CSDV nucleic acid molecules may be used as probes or may be used to draw primer oligonucleotides used in assays such as a polymerase chain reaction (PCR) to detect the presence of CSDV in biological samples, particularly leaves, roots and other plant tissues or organs, such as plants of the genus Citrus and Poncirus. The invention comprises introducing said nucleic acid molecules into cloning vectors and cloning recombinant nucleic acid molecules into cells such as prokaryotes (eg bacteria such as E. coli) and eukaryotes (eg yeast, COS, CHO, and other cells). Cloned CSDV nucleic acid molecules are expressed in cells to provide immunogenic proteins that can be used to produce antibodies against CSDV, which can then be used to detect the presence of CSDV in biological samples. The invention also comprises CSDV nucleic acid molecules, complete or in part, as well as transgenic plants, such as monocotyledons and dicotyledons, containing CSDV nucleic acid molecules in any combination so that their essence increases disease resistance. Sudden Death of Citrus.