尿液中γ-羟基丁酸及其前体物质的检测和应用

摘要

目的：建立尿液中γ-羟基丁酸（gamma-hydroxybutyric acid，GHB）及其前体物质1，4-丁二醇（1，4-butanediol，1，4-BD）和γ-丁内酯（gamma-butyrolactone，GBL）的液相色谱-串联质谱法（LC-MS/MS），为相关案件提供依据。方法以GHB-d6、MOR-d3为内标，尿样经甲醇沉淀蛋白后通过液相色谱分离，电喷雾离子源进行离子化，多反应监测模式对各化合物进行检测。结果 GHB及其前体物质1，4-BD、GBL的检出限分别为0.1、0.1和2μg/mL，准确度为87.6%~98.1%，日内及日间精密度均小于15%，基质效应大于80%。结论所建立的分析方法灵敏度高、简便快速、专属性强、可靠性高，可为司法鉴定实践中涉及GHB的案件提供技术支持和基础数据。%Objective To establish the m ethod to analyze γ-hydroxybutyric acid (GHB ) and its precursors 1,4-butanediol (1,4-B D ) and gam m a-butyrolactone (GBL) in urine through LC-M S/M S and provide evi-dence for related cases. Methods GHB-d6 and M O R-d3 were used as the internal standard. The urine sam ple w as separated by LC after protein precipitation w ith m ethanol. The electrospray ion source w as for ionization. E ach com pound w as detected through m ultiple-reaction m onitoring (M R M ) m ode. Results The lim its of detection of GHB and its precursors 1,4-B D and GBLwere 0.1, 0.1 and 2μg/m L. The accuracy w as 87.6% -98.1% . The intra-day and inter-day precisions were less than 15% and m atrix ef-fects were higher than 80% . Conclusion The m ethod is high sensitive, sim ple, rapid, specific and w ith high reliability. This study has provided technical support and basic data for forensic cases involving GHB .