桃果实总蛋白质双向电泳优化体系的建立

摘要

An effective two -dimensional electrophoresis system was developed to determine the total protein from "huiyulu" peach fruit in this study. The optimum conditions for total protein detection were listed as follows: phenol extraction,800 μg protein sample, 17 cm IPG strip with pH 4-7,and finally detecting proteins with modified colloidal coomassie brilliant blue G-250 staining. With the optimum conditions,a high quality 2-DE map with low background was achieved.%以“晖雨露”水蜜桃为试验材料,建立了一种适用于桃果实总蛋白质的双向电泳体系.最终试验确定采用酚抽法提取桃果实蛋白质,采用17 cm,pH 4～7的线性IPG胶条,800 μg蛋白质上样,并用考马斯亮蓝G-250胶体考染,可以得到背景清晰、分辨率高的双向电泳图谱.对影响双向电泳图谱质量的因素进行了讨论分析.