基于超细剪切细胞破壁技术的藻蓝蛋白提取工艺

摘要

To improve the low efficiency of phycocyanin extracted from Spirulina platensis,the ultrafine sheafing method was applied to break the cell of Spirulina,which will provide the basis of industrial extractionof phycocyanin.The single-factor experiments were conducted to study the effects of liquid-solid ratio,ammonium sulfate saturation,time length of ultrafine shearing on theyield of phycocyanin.The response surface methodology was applied to optimize the above processing parameters.The optimum extraction processing parameters based on ultrafine shearing technology were:liquid to solid ratio:30 mL/g,ammonium sulfate saturation:50％,time length of ultrafine sheafing:15 min.Under the above optimized conditions,the yield of the phycocyanin was 12％.%为解决目前藻蓝蛋白提取工艺效率低下问题,采用超细剪切技术进行螺旋藻细胞破壁,为藻蓝蛋白工业化生产提供理论依据.采用单因素试验方法,研究螺旋藻液料比、硫酸铵饱和度、剪切时间3种工艺参数对藻蓝蛋白得率的影响,并结合响应面试验方法对上述3种工艺参数进行优化.基于超细剪切技术的藻蓝蛋白提取最佳工艺为:液料体积质量比30mL/g,硫酸铵饱和度为50％,剪切时间为15rin,在此条件下得到的藻蓝蛋白得率为12％.