响应面优化重组大肠杆菌表达RGD-TRAIL发酵工艺

摘要

为了提高RGD-TRAIL的表达量,对重组大肠杆菌的发酵工艺进行优化.在单因素实验的基础上,采用Box-Behnken实验设计进行了四因素三水平的响应面分析试验,研究了接种体积分数、pH值、诱导时间、诱导温度对RGD-TRAIL表达的影响.响应面结果表明,RGD-TRAIL表达的最佳条件为:接种体积分数7.88％,pH值7.02,诱导时间10.25 h,诱导温度22℃.在此条件下RGD-TRAIL的表达量达到17.27％,与模型预测表达量(17.31％)接近,较优化前提高了40.06％.%In order to enhance the production of RGD-TRAIL by recombinant E.coli,response surface methodology was applied to optimize the fermentation conditions.Based on the single factor experiments,the inoculation volume,pH value,the induction temperature and induction time were optimized by response surface optimization with 4 factors 3 levels using Box-Behnken experimental design.The optimum conditions for production of RGD-TRAILwere determined as follows:inoculation volume of 7.88％,pH 7.02,the induction time of 10.25 h,induction temperature of 22 ℃.Under the optimal fermentation conditions,the expression of RGD-TRAIL was 17.27％,which was close to the model prediction expression and 40.06％ higher than that before optimization.