PLASMID VECTOR FOR PROMOTING EXPRESSION OF FOREIGN GENE IN ESCHERICHIA COLI, RECOMBINANT ESCHERICHIA COLI, METHOD FOR PRODUCING CHEMICAL SUBSTANCE WITH RECOMBINANT ESCHERICHIA COLI, AND METHOD FOR PRODUCING RECOMBINANT PROTEIN

摘要

PROBLEM TO BE SOLVED: To obtain a plasmid vector which enables the efficient and simple expression of a foreign protein in Escherichia coli.;SOLUTION: The plasmid vector containing a promoter sequence capable of functioning in Escherichia coli cell, an SD (liposome binding) sequence, cloning sites, a medicine-resistant gene, and a replication origin capable of functioning in the Escherichia coli cell is characterized by follows. The cloning sites exist at two positions near to the 5'-side and 3'-side of the SD sequence. The first and second cloning sites contain restriction enzyme-recognizing sequences, respectively, which produce cohesive ends, when cleaved with a restriction enzyme. At least one of the combinations of the restriction enzyme-recognizing sequences contained in the first and second cloning sites is a combination of the restriction enzyme-recognizing sequences which have common cohesive ends and are respectively produced, when cleaved with the restriction enzyme.;COPYRIGHT: (C)2005,JPO&NCIPI