Fermentation process for preparing thymidine by the recombinant E. coli

摘要

A method for producing industrial grade thymidine in E. coli using a metabolic engineering approach is disclosed. The present invention relates to a gene having a nucleotide sequence of SEQ ID NO: 1, deoA , tdk , upp and udp , which are genes involved in a reuse metabolic pathway, is removed, a gene udhA having a nucleotide sequence of SEQ ID NO: 2, yfjB and the SEQ ID NO: gene pspB having the nucleotide sequence described by the three is expressed, carbamoyl-aspartate is Tate gene, purR,pepA and argR relating to the synthesis inhibition is removed and genes that make up the pyr operon (operon) pyrBI,pyrC , pyrD, and pyrE overexpressing the thymidine-producing E. coli strain.