高压液相色谱法同时测定食品中抗坏血酸与异抗坏血酸的含量

摘要

Objective To establish a high pressure liquid chromatography (HPLC) method for simultaneous determination of the contents of ascorbic acid and isoascorbic acid in food. Methods Ascorbic acid and isoascorbic acid were separated using a Venusil HILIC column (250 mm×4.6 mm, 5 μm, 100 Å), taking acetonitrile-0.1% phosphoric acid solution (90∶10, v/v) as the mobile phase and extractant. Results The average recoveries of ascorbic acid and isoascorbic acid were 96.61% and 96.53%. And their minimum detectable concentration was 0.35μg/mL and 0.42μg/mL. Conclusion The method is accurate, sensitive and of precision, and can be used for the determination of ascorbic acid and isoascorbic acid in food.%　　目的建立食品中同时测定抗坏血酸和异抗坏血酸含量的高压液相色谱检测方法.方法用乙腈−0.1%磷酸水溶液(90∶10, v/v)作流动相和提取液,以Venusil HILIC色谱柱(250 mm×4.6 mm,5μm,100Å)分离抗坏血酸和异抗坏血酸.结果抗坏血酸的平均回收率为96.61%,异抗坏血酸的平均回收率为96.53%,异抗坏血酸和抗坏血酸最低检出浓度分别为0.35μg/mL和0.42μg/mL.结论该方法准确、灵敏、精密度高,适用于食品中抗坏血酸与异抗坏血酸的测定.