目的：建立无需衍生化法-超高效液相色谱直接测定食品中的黄曲霉毒素 B1的快速检测方法。方法样品经甲醇-水(7：3, v：v)提取,免疫亲和柱净化, C18色谱柱分离后,采用大体积流通池荧光检测器检测。结果黄曲霉毒素B1在0.10~5.00 ng/mL范围内与峰面积呈良好线性关系, R2>0.999,在饼干、粉丝、点心、沙琪玛、蛋糕、辣椒酱6种基质中,黄曲霉毒素B1在0.50~2.00μg/kg范围内加标,平均回收率在71.7%~111.2%之间,相对标准偏差(RSDs)为3.8%~11.5%,检出限(LOD)为0.05μg/kg。结论本方法灵敏、快速、无衍生、结果准确,能够满足食品中黄曲霉毒素B1残留的检测需求。%Objective To establish a rapid determination of aflatoxin B1 in food by ultra performance liquid chromatography (UPLC) without derivation method. Methods Samples were extracted with methanol water (7:3, v:v), cleaned up by immuno-affinity column, separated by C18 column, and determined by fluorescence detector with large volume flow cell. Results High correlation coefficient (r2>0.999) was obtained in the linear range of 0.10~5.00 ng/mL. The average recovery rates were 71.7%~111.2% at the spiked level of 0.50~2.00 μg/kg and relative standard deviation(RSD) was 3.8%~11.5% in biscuit, vermicelli, refreshment, sacima, cakes, and chili sauce. The limit of quantitation (LOD) was 0.05μg/kg. Conclusion This method is sensitive, rapid, non-derived, and accurate, and it can meet the requirements for determination of aflatoxin B1 in foods.
展开▼
