Objective To establish a method for the rapid detection of casein content in milk powder. Methods The content of casein in infant formula was quantitatively determined by enzyme-linked immunosorbent assay (ELISA). In accordance with the reference of the kit, a standard curve was made, the casein content of three different kinds of samples were calculated, and another 10 kinds of milk powder were detected. Results Casein had a good linear relationship in the range of 0.5～13.5 μg/g, with the correlation coefficient (R2) greater than 0.99. The consistency between detected casein content and label content in 3 kinds of milk powder samples were above 99％. The casein content of 10 kinds of milk powder samples were in the range of 4.17～6.65 g/100 g. Conclusion This method is specific, sensitive, simple, rapid and accurate, which is suitable for the determination of casein content in infant formula.%目的 建立快速检测奶粉中酪蛋白含量的方法.方法 通过夹心酶联免疫吸附法(ELISA)定量测定婴儿配方奶粉中的酪蛋白含量.按照试剂盒内附的操作说明,制作标准曲线,计算3种奶粉样品所对应的酪蛋白含量,同时测量另外10种奶粉样品的酪蛋白含量.结果 酪蛋白在0.5～13.5μg/g内具有良好的线性关系,相关系数(R2)大于0.99.3种奶粉样品实际测得酪蛋白含量与标签上的含量一致性在99％以上.10种奶粉样品酪蛋白含量在4.17～6.65 g/100 g范围内.结论 该方法专一性强,灵敏度高,简便、快速、准确,可用于婴儿配方奶粉中的酪蛋白含量的测定.
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