细胞周期相关因子cdtl和geminin在急性白血病患者中的表达

摘要

The purpose of this study was to detect the expression levels of geminin and cdtl in peripheral blood and bone marrow from patients with newly diagnosed acute leukemia(AL),and further explore effects of them in the patho-genesis of AL.mRNA expression of geminin and cdtl in peripheral blood and bone marrow of newly diagnosed AL patients was detected by SYBR Green real-time quantitative reverse transcription polymerase chain reaction(SYBR-RT-PCR).The results showed that mRNA expressions of both geminin and cdtl in peripheral blood were positive in 10 out of 13 newly diagnosed ALL patients(76.92%)and in 9 out of 14 newly diagnosed AML patients(64.29%),while no positive expression of these 2 genes was detected in 10 normal controls:mRNA expression levels of geminin and cdtl in bone marrow of newly diagnosed ALL and AML,pailents were 108.06±67.34 and 52.37±35.16.62.66±58.69 and 26.68±22.29,respectively,which were higher than those in normal controls(11.81±2.83 and 7.32±5.77),there were significant differences(p＜0.01 and p＜0.05).mRNA expression of geminin was significantly positive related to mRNA expression of cdtl in bone marrow of 34 newly diagnosed AL patients(r=0.55,p＜0.01).It is concluded that mRNA expressions of geminin and cdtl are enhanced and significantly positively related between them in bone marrow of AL patients.The over-expression of geminin and cdtl mRNA may play an important role in pathogenesis of AL.%本研究旨在检测geminin和cdtl在初诊急性白血病(AL)患者外周血或骨髓细胞中的表达水平,并进一步探讨其在AL发病机制中的作用.采用SYBR Green实时定量逆转录聚合酶链反应(SYBR-RT-PCR)技术,检测初诊AL患者外周血或骨髓细胞geminin和cdtl mRNA的表达.结果表明:13例ALL初诊患者外周血中有10例孪蛋白(geminin)和cdtl mRNA表达阳性,阳性率为76.92%;14例AML初诊患者外周血中有9例表达阳性,阳性率为64.29%;而正常对照组中无阳性表达(0/10).ALL和AML初诊患者骨髓细胞中geminin和cdtl mRNA表达(ALL:108.06±67.34,52.37±35.16;AML;62.66±58.69,26.68±22.29)均高于对照组(11.81±2.83,7.32±5.77),差异有统计学意义(p＜0.01,p＜0.05);34例初诊AL患者骨髓细胞中geminin和cdtl mRNA的表达呈明显的正相关(r=0.55,p＜0.01).结论:geminin与cdtl在初诊AL患者中表达增高,且两者在AL骨髓细胞呈正相关,其过度表达可能在AL的发病机制中起重要作用.